Solvent to Break Streptavidin-Biotin Interaction

March 10, 2020

Harvard Medical School
Cell Biology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

1,1,1,3,3,3-Hexafluoro-2-propanol

Catalog Number:

52517-10X1ML

This reagent is very powerful to elute biotinylated proteins from streptavidin beads

Experimental Design and Results Summary

Application

Elution of biotinylated proteins from streptavidin beads

Starting Material

N/A

Tips

Must use this in the hood as the solvent is very volatile

Results Summary

This solvent always works perfectly to strip all the biotinylated proteins captured by streptavidin beads

DOI or PMID #

N/A

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Summary

The Good

Powerful

The Bad

Nothing

The Bottom Line

This is such a useful solvent for biological experiments.

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