Excellent Antibody for Mouse CXCR5 Staining

April 02, 2020

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

PE anti-mouse CD185 (CXCR5) Antibody

Catalog Number:

145503

Clone Number:

L138D7

I used this antibody to stain CXCR5+Foxp3- follicular helper and CXCR5+Foxp3+ regulatory T-cells in mouse. This antibody stained well and showed distinguished populations when used in combination with Foxp3 antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse splenocytes and lymph node cells

Primary Incubation

1:100 for 90 min at 4 degree

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This antibody stained well and showed distinct population. I am able to get reproducible results.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Ease of optimization and reproducible results

The Bad

Nothing noted

The Bottom Line

It's worth buying this antibody

Share your experience with other scientists. Write a Review! »

Join the discussion