SuperFect Transfection Reagent for Plasmid DNA Transfection

March 09, 2020

University of Alabama at Birmingham
Pathology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

SuperFect Transfection Reagent

Catalog Number:

301305

Human breast cancer cell lines were transfected with plasmid DNA using the SuperFect Transfection Reagent. The plasmid DNA contained a target protein sequence and GFP to assess transfection efficiency.

Experimental Design and Results Summary

Application

Transfection

Starting Material

Human breast cancer cells and plasmid DNA

Tips

Follow the protocol carefully, timing may need to be optimized

Results Summary

Levels of GFP indicate the plasmid DNA was successfully transfected into the cells and the efficiency was sufficient.

DOI or PMID #

N/A

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Summary

The Good

Fast, convenient for transient transfections

The Bad

N/A

The Bottom Line

SuperFect Transfection Reagent is an adequate alternative to Lipofectamine for transfecting plasmid DNA. Some plasmids may be more effectively transfected and for longer duration compared to the popular Lipofectamine.

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