Works Well to Detect Human c-Jun

March 06, 2020

Pharmacology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

c-Jun

Catalog Number:

9165

Clone Number:

60A8

This antibody worked well to detect human c-jun full length from MDA-MB-231 cell extracts.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MDA-MB-231- human

Primary Incubation

1:500

Blocking Agent

Normal Donkey Serum

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

Overall with minimal optimization this antibody worked well to detect c-jun. There was however a non specific band in the higher kDa region.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

One non specific band

The Bottom Line

Works well for detection.

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