Activation of JNK2 by Infection with Mycobacterium

SCHOOL OF VETERINARY MEDICINE AND BIOMEDICAL SCIENCES
University of Nebraska
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

JNK2 Antibody

Catalog Number:

SC-572

Clone Number:

D-2

c-Jun N-terminal protein kinase (JNK) (also known as stress-activated protein kinase [SAPK]) (8) is a subfamily of the mitogen-activated protein kinase (MAPK) superfamily. Here we performed our study infection with mycobacterium can activate JNK2 protein.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Animal tissue cells, RAW 264.7 cells

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skim milk

Secondary Incubation

1:5000 dilution in 5% skim milk prepared in 1X TBST

Tertiary Incubation

N/A

Detection

ECL

Results Summary

We found that infection with mycobacterium increased level of JNK2 using Santa Cruz antibody.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Very good signal no background

The Bad

None

The Bottom Line

This antibody is very good. Highly recommended.

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