Using GAPDH as a Reference Gene

February 28, 2020

SCHOOL OF VETERINARY MEDICINE AND BIOMEDICAL SCIENCES
University of Nebraska
Postdoctoral Researcher

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Company:

GeneTex

Product Name:

GAPDH Antibody

Catalog Number:

GTX627408

Clone Number:

GT239

glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) has been frequently considered as constitutive housekeeping gene for Western blot and used to normalize changes in specific gene expressions. However, these expressions have been shown to be affected by the sample type and experimental conditions. We investigated which housekeeping gene is useful for our study.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate and tissue lysate

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skim milk

Secondary Incubation

1:5000 dilution in 5% skim milk prepared in 1X TBST

Tertiary Incubation

N/A

Detection

ECL

Results Summary

We performed different experiments in disease condition and control group. We got good results by using GeneTex GAPDH as a reference gene (housekeeping gene).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good signal

The Bad

Nil

The Bottom Line

Western blot is used to identify changes in specific gene expressions. However, these expressions have been shown to be affected by the sample type and experimental conditions. We investigated which housekeeping gene is useful for our study. We got good result by using GeneTex GAPDH as a reference gene (housekeeping gene).

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