Detection of Apotosis and Necrosis

University of Nebraska
SCHOOL OF VETERINARY MEDICINE AND BIOMEDICAL SCIENCES
Postdoctoral Researcher

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Company:

Promega

Product Name:

Real time Glo Annexin V apoptosis and Necrosis

Catalog Number:

JA1011

The RealTime-Glo Apoptosis and Necrosis Assay measures exposure of phosphatidylserine (PS) on the outer leaflet of the cell membrane during the apoptotic process, and loss of membrane integrity during secondary necrosis. Annexin V binding is detected with a simple luminescence signal, and necrosis is detected with a fluorescent DNA binding dye. Here we performed whether cells undergoing apoptosis or Necrosis we got good result mycobacterium infection cells undergoing apoptosis by using Real time Glo annexin V and necrosis kit

Experimental Design and Results Summary

Application

Spectramax molecular device

Starting Material

Cells and mycobacterium

Tips

Light sensitive we have to protect from light

Results Summary

Here we performed whether cells were undergoing apoptosis or necrosis. We got good results in mycobacterium infection cells undergoing apoptosis by using Real time Glo annexin V and necrosis kit

DOI or PMID #

N/A

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Summary

The Good

It works very well and has very easy detection methods

The Bad

Light sensitivity

The Bottom Line

Very useful and very easy method for detection of apoptosis and necrosis by using Promega real time glo annexin and necrosis kit.

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