Great GFAP Antibody from Thermo Fisher

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

GFAP Monoclonal Antibody

Catalog Number:

13-0300

Clone Number:

2.2B10

Our research focuses on understanding a role played by glial cells in neurodegenerative diseases. The specific goal of this experiment was to evaluate astrocyte activation in the course of disease progression. This antibody demonstrated excellent staining for astrocytes, in the optic nerve.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Feline optic nerve tissues

Primary Incubation

Diluted at 1:2000, overnight at 4°C

Blocking Agent

10% normal donkey serum + 0.5% triton X + 0.1M PBS

Secondary Incubation

Alexa 488 conjugated anti-rat IgG 1:500, 1 hr at RT

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Robust signals from the labelling for astrocytes was detected (green) in feline optic nerve tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Highly specific with no background

The Bad

N/A

The Bottom Line

This is a very specific astrocyte marker for this species and generates robust signals.

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