Western Blot for NRF1 with Santa Cruz Antibody

Cellular/Molecular Biology
Texas Tech Health Sciences Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Antibody

Product Name:

NRF1

Catalog Number:

SC-23624

Clone Number:

147.1

Transcription factor Nrf1 acts as a unique vital player in maintaining cellular homeostasis and organ integrity during normal development and growth throughout the life process. Loss-of-function of Nrf1 results in severe oxidative stress, genomic instability, embryonic lethality, developmental disorders. Here we checked the time course effect of NRF-1 by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell and mouse tissue lysate samples

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skimmed milk

Secondary Incubation

1:4000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

Our NRf1 protein expression very good signal and time dependent decreases NRF1 protein expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion