Thermo Fisher Scientific
CD289 (TLR9) Monoclonal Antibody (eB72-1665), PE, eBioscience™
12-9099-82
eB72-1665
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I stained mouse PBMCs with this antibody to detect the expression of TLR9 on B cells. I gated on B cells using CD3, CD45 and B220 antibody.
Flow Cytometry
Mouse Peripheral blood monocytes cells
1:100 at 4 degrees for 30 minutes
2% FBS in PBS
Not applicable
Flow cytometry
The antibody worked well and detected the expression of TLR9 on B lymphocytes.
N/A
No background
It is expensive than other companies such as BioLegend.
This antibody detects the levels of TLR9 and shows high specificity and sensitivity.