Working CD340 Antibody from BioLegend

January 31, 2020

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-human CD340 (erbB2/HER-2) Antibody

Catalog Number:

324405

Clone Number:

24D2

I detected the expression of Her2 on the surface of breast cancer cell line MDA-MB-231 using this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

MDA-MB-231 cells

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This cell line does not express HER2 protein. This antibody specifically binds to HER2 and did not detect it on MDA-MB-231.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No non-specific staining

The Bad

Nothing

The Bottom Line

It's a great antibody to detect HER2/CD340 expression.

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