Great Staining and Use of a Dim Fluorophore

February 05, 2020

Surgery
NYU Langone Medical Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-mouse CD8a Antibody

Catalog Number:

100722

Clone Number:

53-6.7

Straightforward staining procedure with clear separation of CD8+ T cell population. The antibody is easy to use with clear resolution.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine pancreas with orthotopically injected murine pancreas cancer cells

Primary Incubation

Incubated at 4C for 1h, dilution 1:500

Blocking Agent

1% FACS, anti-CD16/CD32, Mouse serum, Rat IgG

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT

Results Summary

Clear separation of CD8+ population at low laser voltage.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Straightforward staining procedure, good use of dim fluorophore, nice separation of populations

The Bad

Natural limitations of tandem dies

The Bottom Line

Easy-to-use antibody with clear resolution

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