MilliporeSigma
H2B
07-371
Polyclonal
Browse Similar Products: H2B Western Blot Antibodies
The SAGA complex is responsible for the cleavage of monoubiqutin from histone H2A abd H2B. Here, I assess the global levels of H2B through WB following suppression of a component of the SAGA complex in OCI AML2 cells.
Western Blot
AML2 whole cell lysate
1:1000 RT 1hr in 5% skim milk
5% milk - 10 min room temperature
5% milk - 30 min room temperature secondary Ab 1:10K
N/A
ECL - film
Good antibody for western blot with minimal background. Approx. size 17 kDa. Conditions represent samples following CRISPR/Cas9 deletion of Gene X vs. a control. Vinculin is shown as the loading control.
Good Antibody for quickly assessing global H2B levels by WB
Good antibody that works well!