A Good VDUP1 Western Blot Antibody

Cellular/Molecular Biology
Texas Tech Health Sciences Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology, Inc.

Product Name:

VDuP1

Catalog Number:

sc-271238

Clone Number:

H-12

Polydatin prevents fructose-induced liver inflammation and lipid deposition through increasing miR-200a to regulate Keap1/Nrf2 pathway. This antibody is highly specific for VDUP.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1x TBST

Blocking Agent

5% skim milk

Secondary Incubation

1:4000 5% milk

Tertiary Incubation

None

Detection

ECL

Results Summary

Novel pathological mechanism of fructose-induced redox status imbalance and suggest that the enhancement of miR-200a to control Keap1/Nrf2 pathway by polydatin is a therapeutic strategy for fructose-associated liver inflammation and lipid deposition.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background and strong signal

The Bad

None

The Bottom Line

This antibody is highly specific and works great for western blot. Highly recommended.

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