A Good IL-18 Antibody from Western Blot

Cellular/Molecular Biology
Texas Tech Health Sciences Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

EMD Millipore

Product Name:

Interleukin-18 (IL-18) Antibody

Catalog Number:

06-1115

Clone Number:

Polyclonal

Browse Similar Products: IL-18 Western Blot Antibodies

IL-18 has been shown to be a pro-inflammatory cytokine that induces interferon-gamma. IL-18 has also been found to have the ability to activate nuclear factor-kappaB (NFkB), express Fas ligand, induce CC and CXC chemokines, and also has been found to increase the production of viable human immunodeficiency virus. IL-18 has been linked to syndromes displaying symptoms.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1x TBST

Blocking Agent

5% skim milk

Secondary Incubation

1:4000 5% milk

Tertiary Incubation

None

Detection

ECL

Results Summary

IL-18 also seems to have some control over homeostasis and behavior in the central nervous system as well as neuroinflammatory activity.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

No background and strong signal

The Bad

None

The Bottom Line

This antibody is highly specific and works great for western blot. Highly recommended.

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