Thermo Fisher Scientific
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568
A-11011
Info:Supplier Page View Product Specs View Product
Mouse was sacrificed, collected the liver, fixed, sections were made, blocked and probed with on target rabbit-primary antibody. After overnight incubation in primary antibody, sections were washed and probed with Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 at a dilution of 1:1000 in 3% BSA solution in PBS. After a 1-hour incubation, the secondary antibody was removed, sections were washed in PBS, mounted in gold-antifade mounting medium and examined under a fluorescent microscope using the green filter.
Double Immunofluorescence staining of tissue samples
Mouse tissue, Primary antibody
Avoid drying of the reagent in the bottle.
A specific and bright red signal from Alexa Fluor 568 was observed in the tissue section and captured using a microscopic color camera. The intensity of Alexa fluor was not bleached over the exposure time.
None
A highly specific secondary antibody conjugated with Alexa Fluor 568
Best secondary antibody