Sigma-Aldrich
Anti-Heat Shock Protein 70 (HSP 70) Antibody
AB9920
Polyclonal
Browse Similar Products: HSP 70 Western Blot Antibodies
To study the role of heat shock protein during fungal toxin induced oxidative stress, HSP 70 protein was detected in control and toxin exposed tissue samples using this antibody. Briefly tissue lysate was prepared in tissue lysis buffer, maintaining cold chain during the process. Protein concentration in tissue lysate was quantified and 50 µg of protein was resolved on 10% SDS-PAGE, transferred to nitro-cellulose membrane, blocked and probed with anti-HSP 70 primary antibody overnight. Again after multiple washing of the membrane, probed with anti-rabbit HRP conjugated secondary antibody for 1 h. HSP 70 protein expression levels detected on X-ray flim with chemiluminescent detection reagent.
Western Blot
Mouse liver tissue lysate
1:4000 in 5% skimmed milk, 0.05% Tween 20 and 1X PBS at 4 degree C, overnight with gentle shaking
5% skimmed milk, 0.1% Tween 20 in PBS for 1 h
1:10,000 anti-rabbit HRP conjugated secondary antibody
None
Chemiluminescence detection reagent on X-ray film
Sharp single protein band of nearly 70 kDa was observed on the X-ray film corresponding to HSP-70 protein in control and toxin exposed liver tissue samples.
N/A
Good antibody