Sensitive PE/Cy7 Anti-Mouse GM-CSF Antibody from BioLegend

Innate Immunity
DRFZ
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-mouse GM-CSF Antibody

Catalog Number:

505411

Clone Number:

MP1-22E9

The aim of our research is to check the different expression profiles, in terms of cytokines and surface markers, of in vitro differentiated CD4+ T cells. This antibody was used in order to identify GMCSF expression of in vitro differentiated naive T cells towards Th1 phenotype

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

CD4+ murine T cells

Primary Incubation

15mins in 4°C

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

Good, sensitive, and high-resolution antibody to detect GM-CSF production in cultured T cells after stimulation with PMA/Ionomycin.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Good, sensitive, high resolution

The Bad

No bad things about it

The Bottom Line

I would recommend this antibody

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