Time Course Effect of Anti-Interleukin-18 by Chemoattractant

January 02, 2020

Department of Pharmacology and Toxicology
Augusta University
Postdoctoral Researcher

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Company:

MilliporeSigma

Product Name:

Anti-interleukin-18

Catalog Number:

06-1115

Clone Number:

Polyclonal

Browse Similar Products: Interleukin-18 Western Blot Antibodies

Nicotinic acid is converted into nicotinamide adenine dinucleotide (NAD), which allows for the multitude of redox functions met by NADþ, NADH, NADPþ, and NADPH in metabolism. Here we checked the time course effect of Anti-interleukin-18 by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Rat tissue lysate

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skimmed milk

Secondary Incubation

1:4000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent increase of Anti-interleukin-18 by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody is very specific and the signal is strong. Highly recommended.

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