Good for Mass Spec Sampling

Seoul National University
Biology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

2-Chloroacetamide

Catalog Number:

C0267

I use this to methylate cysteines when preparing protein samples for mass spec.

Experimental Design and Results Summary

Application

Mass spec sampling

Starting Material

HEK293T cell lysates

Tips

I used 20 mM as final condition, freshly prepared, and incubated cell lysates with chloroacetamide for 15min at room temperature.

Results Summary

This is a good quality chemical for cysteine methylation

DOI or PMID #

N/A

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Summary

The Good

High quality

The Bad

Nothing

The Bottom Line

I recommend this to others who prepare proteomic samples.

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