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PE/Dazzle™ 594 anti-mouse/human CD11b
101256
M1/70
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B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B lineage cells during autoimmunity. In this experiment, I use anti-CD11b to stain B1 cells and macrophages from the peritoneal cavity
Flow Cytometry
Wild-type mouse peritoneal cavity lavage
Surface staining with anti-CD11b (1/4000) (20 min, 4° in the dark)
Clone 2.4G2 (Fc block)
N/A
Flow cytometry, yelllow green laser
I could stain macrophages expressing high level of CD11b (red peak) and CD11b+ lymphocytes that are B1 cells (lower blue peak) both from peritoneal cavity
Antibody works very well and is bright enough to allow distinguish B1 cells that express
Nothing bad
It is an excellent option as it can be used at 1/4000