Guava Nexin Reagent for Flow Cytometry - 100 Tests

November 27, 2019

Drexel University
Pediatrics
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

Guava Nexin® Reagent (100 tests)

Catalog Number:

632844

The Guava Nexin Reagent is a mix-and-read assay for monitoring externalization of phosphatidylserine (PS) through the binding of Annexin V to the exposed PS. I have used this reagent for measuring levels of Annexin V in our control and test samples. Our results was good and showed difference between the group.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

HEK Cells

Tips

Cells should not be clumped while harvesting. Reagent must be protected from the light.

Results Summary

This is a sensitive assay. Our lab has expertise in FACs, It worked well and got satisfactory results.

DOI or PMID #

N/A

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Summary

The Good

Very sensitive reagent and ready for use.

The Bad

None

The Bottom Line

This is sensitive reagent so standardization may be needed. otherwise see the background in the FACs. Cells should not be clumped. This reagent offers a simple, reproducible, and sensitive assessment of early apoptosis. I recommend for those working to see the apoptosis in live and healthy cells.

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