Santa Cruz Biotechnology
p53 Antibody (DO-1)
sc-126
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This antibody recognizes endogenous levels of total p53 protein. I have used this in mice lung lysates. I got a clear very specific band in my W. Blots.
Western Blot
Mice lung lysate
1:500, overnight on the shaker
3% BSA
1:2000, 1hr at room temp
N/A
Licor imaging
An specific single band at 53kDa. Washing is very important to minimize background.
Washing between the incubation is very important to minimize background
Very good
None
This a one of the very antibody in Santa Cruz to work with. I recommend for users working in this field.