Pan Phosphatase Inhibitor Cocktail

Seoul National University
Biology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma (Roche)

Product Name:

PhosSTOP™

Catalog Number:

4906837001

Very convenient cocktail for cell lysis.

Experimental Design and Results Summary

Application

Phosphatase inhibition upon cell lysis

Starting Material

Human tissue culture cell line HEK293T and HCT116

Tips

works well

Results Summary

I use this for mTOR signaling pathway monitoring using WB method. This is a bit pricy but works quite well compared to sodium pyrophosphate/b-glycerphosphate.

DOI or PMID #

N/A

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Summary

The Good

Effective blocking of phosphatase

The Bad

Pricy

The Bottom Line

Great for very important experiments such as phospho-enrichment Mass spec.

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