Novex® NuPAGE® Pre-cast Protein Gels by Invitrogen

Novex® NuPAGE® Pre-cast Protein Gels by Invitrogen
Novex® NuPAGE® gel system by Invitrogen is a revolutionary neutral pH system for polyacrylamide mini-gel electrophoresis. A neutral pH means increased stability of proteins and gels, providing with increased confidence in electrophoresis and blotting results. Novex® NuPAGE® pre-cast protein gels provide the easiest solution to routine protein electrophoresis. They are manufactured in a variety of thicknesses and formulations suitable for separation of proteins of variable sizes. NuPAGE® Bis-Tris and Tris-Acetate gels are suitable for denaturing gel protein electrophoresis. NuPAGE® Tris-Glycine gels can be used for both native and denatured proteins. Pre-made running buffer (either native PAGE or SDS-PAGE, MOPS- and MES-based) can be purchased as a 10X concentrate to be diluted prior to use. To accommodate different sample volume requirements, gels are available in eight different well formats: From a single well to up to 17 wells, and two gel thicknesses. Each gel is individually wrapped in a plastic pouch and sold in boxes of ten gels. Gel packs are stored in the refrigerator and most types of gels have a shelf life of up to one year. Pre-made mini gels provide the most significant advantage, providing reproducible and reliable separation of proteins into well-resolved bands without spending loads of valuable time casting gels. The other advantages are the ease of use and assembly, and availability of the whole system (XCell SureLock™ Novex® Mini-Cell) to easily run these gels.

I ran Novex® NuPAGE® pre-cast protein gels using the XCell SureLock™ Novex® Mini-Cell system, manufactured by Invitrogen, Inc. The system includes buffer core, lower buffer chamber, gel-tension wedge, square plastic dam and the lid with the cords, which can be directly connected to any power supply. The set-up procedure was very easy with no clamps and/or screws. When I became familiar with the system, it took me less than 5 min to rinse the gel, assemble it on the apparatus, load the samples, and start the run. Handling the gels after the run is a bigger challenge because of their thinness, but following the instructions helped me avoid major breakage. Before our lab purchased these gels, I had done an extensive cost analysis and found the pricing to be very competitive.

I performed both native and denaturing protein and protein-DNA electrophoresis using both NuPAGE® Bis-Tris and Tris-Glycine gels of 1 mm thickness with 6-10 wells each. I analyzed protein extracts from lymphoblasts of patients and control samples looking for differences related to the disease. I did a number of separate, nearly identical runs and was very pleased to see exceptional run-to-run consistency. It is important to note, however, that because of their small size, these gels will only be suitable for applications which include analyzing proteins and DNA-protein complexes of small to medium molecular size.

In summary, my experience suggests that Novex® NuPAGE® pre-cast protein gels by Invitrogen are definitely a good choice for vertical mini-gel protein electrophoresis for a variety of applications.

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Novex® NuPAGE® Pre-cast Protein Gels by Invitrogen
The Good

Easy to run, easy to handle, sharp bands, and great reproducibility.

The Bad

Small size incomparable with some of the applications.

The Bottom Line

Pre-made protein mini-gels with consistently reliable performance.