This BioLegend Antibody Stains PD-L1+ Cells Well

November 01, 2019

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 711™ anti-mouse CD274 (B7-H1, PD-L1) Antibody

Catalog Number:

124319

Clone Number:

10F.9G2

We wanted to study PD-L1 expression in the diseased vs healthy state, so we used the antibody to stain for PD-L1 expression in mice.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells stimulated with anti-CD3

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stains PD-L1+ cells very clearly

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody is great for identifying PD-L1+ cells

The Bad

None to mention

The Bottom Line

The antibody is very useful for staining for PD-L1 in mice.

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