Spin Column Phospho-Peptides Enrichment

Havard Medical School
Dpt of Cell Bio
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

High-Select™ Fe-NTA Phosphopeptide Enrichment Kit

Catalog Number:

A32992

Spin column used to purify phospho-peptide from digested protein lysate prior to mass spectrometry analysis.

Experimental Design and Results Summary

Application

Phospho-peptide enrichment

Starting Material

Trypsin digested HeLa lysate

Tips

None, just follow the provided protocol

Results Summary

Routinely achieve >96-98% enrichment of phospho-peptides.

DOI or PMID #

N/A

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Summary

The Good

Ease of use. Good enrichment

The Bad

None

The Bottom Line

These spin columns are easy to use and give very good results.

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