MilliporeSigma
Oligomycin A
75351
This compound was used at 5 uM to depolarize mitochondria from HeLa cells and induce activation of the PINK1/PARKIN pathway.
Induce activation of PINK1/PARKIN
HeLa cells
Use in combination with 10 uM antimycin A, for even more robust activation
Robust activation of pathways, as early as 30 to 1h minutes post treatment, as judge by stabilization and accumulation of PINK1.
10.1016/j.molcel.2014.09.007
Works well across cell lines
Expensive
Works very well across cell lines, even in induced-neurons (see example). This is our go-to method to activate the PINK1/Parkin pathway.