Cell Signaling Technology
phospho-S6 ribosomal protein Ser253, 236
4858
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I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. This Phospho-S6 Ribosomal Protein antibody is really good. One of the best.
Western Blot
293T, HCT116 cells
1:2000 dilution in 5% BSA, TBST. overnight at 4C
5% non-fat milk in TBST
1:20000 Odyssey IRDye800 (rabbit)
Wash TBST, 5min, 3 times
Odyssey Clx
This antibody is very strong and specific. There is a thin band (off target) above the pS6, but overall specificity is very high.
N/A
Very good.
This antibody is one of the best antibodies I have ever used.