Cell Signaling Technology
ATG5
12994
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Browse Similar Products: ATG5 Western Blot Antibodies
I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. This ATG5 antibody works very well just like many other CST antibodies.
Western Blot
293T
1:1000 dilution, O/N
5% non-fat milk
1:20000 odyssey IRDye800, 1h
Wash with TBST three times
Odyssey clx
This antibody works very well and was used for KO screening. Clear KO was then validated by next-generation sequencing. Highly recommend!
10.1016/j.molcel.2019.03.034
N/A
Specificity
Slight amount of background
Good!