BioLegend CD4 Antibody Works Great!

August 14, 2019

Internal Medicine
East Tennessee State University
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

APC anti-human CD4 Antibody

Catalog Number:

300537

Clone Number:

RPA-T4

We want to see the CD4 expression in human PBMCs, PBMCs were purified from whole human blood, cultured for 1 day, then stained CD4-APC for 20min, washed with isolation buffer, applied on Flow Cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:50, isolation buffer

Blocking Agent

Isolation buffer

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD C6 Plus

Results Summary

CD4 express cell population ranges from 20%-70%.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Separate well!

The Bad

None found.

The Bottom Line

Good product, easy to analyze!

Share your experience with other scientists. Write a Review! »

Join the discussion