Good Dimethyl-Histone H3 Antibody from Sigma

February 13, 2020

Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

Anti-dimethyl-Histone H3 (Lys27) Antibody

Catalog Number:

07-452

Clone Number:

Polyclonal

I used this antibody to detect the expression of H3K27me2 in mouse cad cell line by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Cad cells

Primary Incubation

1:2000, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:2000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

The band is very specific but it takes a little bit longer time to see the signal.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody is specific

The Bad

N/A

The Bottom Line

I recommend using this antibody to detect the expression of H3K27me2.

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