Promega
GoTaq® Green Master Mix
M7122
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We use this PCR master mix for colony PCR reaction from bacterial colony. We selected this product because it's easy to use without doing any optimization; just add primers, water, and direct bacterial colony without disrupting bacterial cell. It works well for my all colony PCR so far and I have been using it for one year.
Colony PCR
Bacterial Cell
Cell mass should be less
Specific band for PCR product
N/A
Specific band
Best kit I have used so far for colony PCR.