GFP Antibody from Thermo Is Very Good

August 05, 2019

Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

GFP Polyclonal Antibody

Catalog Number:

A10262

Clone Number:

Polyclonal

My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. In this experiment, I need to check the expression of GFP after transfecting GFP plasmids in cultured dorsal root ganglion cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse dorsal root ganglion cell culture

Primary Incubation

1:1000, overnight at 4°C

Blocking Agent

PBST (0.3%) containing 10% goat serum

Secondary Incubation

1:500, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

CCD camera connected to a Zeiss inverted fluorescence microscope controlled by AxioVision software

Results Summary

This antibody is sensitive and specific.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody works well.

The Bad

N/A

The Bottom Line

I definitely recommend this antibody.

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