Staining Microglia Cells In Vitro (Novus)

August 14, 2019

Molecular Biology and Biochemistry
Medical University of Graz
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

CD11b Antibody (M1/70.15)

Catalog Number:

NB600-1327

Clone Number:

N/A

The antibody was used in order to stain primary murine microglia for confocal microscopy.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Primary murine microglia

Primary Incubation

1/100, O/N at 4°C

Blocking Agent

5% BSA

Secondary Incubation

Cy3, 1/300, 30min at 4°C

Tertiary Incubation

N/A

Detection

Confocal Microscopy

Results Summary

Treatment with LPA (1µM) for 24 hours induces changes in m,icroglia morphology.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use and works as expected

The Bad

None

The Bottom Line

A good antibody for microglia visualization.

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