ß-Actin: House Keeping Gene for Western Blot

Pediatrics
Drexel University
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

β-Actin (C4)

Catalog Number:

sc-47778

Clone Number:

C4

Beta-actin is one of the reliable house keeping gene.In our lab we used this antibody for Western blots. I found this antibody is easy to optimize and gives very clear and crisp band of 43 kDa. This antibody can be used for wide variety of samples such as human, mice, rabbit, monkey and others. I have used for human and mice origin samples.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lung cell line

Primary Incubation

β-Actin (1:1000 dilution), Overnight at 4 degree

Blocking Agent

Odyssey blocking buffer (1:1) ratio with 1x PBS buffer

Secondary Incubation

Goat anti-Mouse IgG, LICOR (1:5000 dilution) at RT for 1hr

Tertiary Incubation

N/A

Detection

Scanned on Odyssey® CLx (auto scan 700 & s800).

Results Summary

Very good results as it appears in the images.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very good

The Bad

N/A

The Bottom Line

I would highly recommend this antibody for internal control for Western blots.

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