Tocris Bioscience
8-Bromo-cAMP, sodium salt
1140
Info:Supplier Page View Product Specs View Product
Cyclic AMP stimulates CaMKII phosphorylation leading activation of the phospholamban-SERCA2a axis. To investigate this, we used 8-bormo-cAMP.
Cell culture
HL-1 cardioymocytes
NA
HL-1 cardiomyocytes were treated with 100 nM fostriecin, 100 nM Okadaic acid or 50 nM 8-bromo-cAMP for 30 min followed by Western blot analysis for pCaMKII expression.
N/A
Works as expected.
8-bromo-cAMP increased phosphorylation of CaMKII; indicating role of cAMP in CaMKII dephosphorylation.