Easy-to-Use Cytotoxicity Assay from Promega

Charité-Universitätsmedizin Berlin
Neuropathology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Promega

Product Name:

CytoTox 96® Non-Radioactive Cytotoxicity Assay

Catalog Number:

G1780

The cytotoxicity assay offered by Promega is very easy to use, quickly done and is very reliable.

Experimental Design and Results Summary

Application

Cytotoxcity of neontal microglia or astrocytes

Starting Material

Supernatant of cultured neonatal microglia or astrocytes from mice

Tips

As positive control, cells can be lysed with lysis buffer provided or with TritonX-100

Results Summary

Easy to quantify LDH release by cells. Assay can be performed in 45min. Analysis is very straight forward.

DOI or PMID #

N/A

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Summary

The Good

Quick and easy protocol

The Bad

Positive control provided in the kit has to be diluted 1:2 to show a positive signal.

The Bottom Line

Very good kit for fast quantification of LDH release of cultured cells!

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