Cell Signaling Technology (CST)
Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody
9464
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Our lab studies the role of mTOR signaling in T cell such as mTORC1 and mTORC2 signaling during T cell differentiation and function. With immunoblot analysis, we assess mTORC2 activity in WT T cells upon T cell activation. Foxo proteins are a downstream node of mTORC2; therefore, we measure phosphorylation of these proteins to assess mTORC2 signaling.
Western Blot
Activated murine CD8+ T cells
1:2500 in 4%BSA/TBST 4 deg overnight with shaking
5% dry milk/TBST 1 hr room temperature with shaking
1:5000 anti-Rabbit HRP (CST) in %5 milk/TBST 1 hr room temp with shaking
None
UVP Imaging System
We are able to detect p-Foxo1/3 activity in our T cell lysates. The signal is quite strong when cells were stimulated with TCR activation.
N/A
Probe with total Foxo1 antibody to ensure correct band.
Quite robust of a signal and can use diluted antibody multiple times.
One of the few antibodies available to truly assess p-Foxo1/3 activity. Very widely used by scientists.