Cell Signaling Technology
Phospho-FoxO1 (Ser256) Antibody
9461
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Our lab studies the role of mTOR signaling in T cell such as mTORC2 signaling during T cell differentiation and function. With immunoblot analysis, we assessed mTORC2 activity using pFoxo1 (S256) as a readout in WT T cells upon T cell activation.
Western Blot
Murine CD8+ T cells
1:1000 in %4 BSA/TBST 4 deg overnight shaking
5% milk/TBST room temp shaking
1:5000 anti-Rabbit HRP (CST) in 5%milk/TBST room temp 1 hr shaking
None
UVP Imaging System
We are able to detect differential p-Foxo1 activity in our conditions. The signal is quite okay since exposure time was low but this is based on our conditions and samples.
N/A
Dilute as needed since polyclonal
Bands are sharp and no non-specific bands
One of the few antibodies on the market to measure p-Foxo1 (S256) activity through western blotting.