Excellent Quantification In Vitro and In Vivo

April 16, 2019

Pharmaceutical Sciences
Pharmaceutical Sciences
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Boster Biological

Product Name:

Mouse TNF Alpha PicoKine™ ELISA Kit

Catalog Number:

EK0527

I used this kit to quantify the level of pro-inflammatory cytokine TNFa in vitro in cell culture media and in vivo in mouse serum. It gave me reliable reproducible results in both the experiments.

Experimental Design and Results Summary

Application

TNFa quantification in vitro and in vivo

Starting Material

Cell culture media, mouse serum

Tips

Do a test run of sample and quantities may be higher than the standard plot range.

Results Summary

I was able to estimate TNFa in cell culture media before and after treatment with lipopolysaccharides in primary macrophage cell line. In vivo estimation was done in control and treated group of mice (high fat diet treatment) by collecting blood, separating serum, and estimating the cytokine levels in serum. Overall reliable estimation was achieved.

DOI or PMID #

N/A

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Summary

The Good

Reliable reproducible result and brand of ELISA kits

The Bad

Long incubation time, chances of error due to similar bottles of reagents in all kits

The Bottom Line

Good ELISA kit. Reliable vendor. Good continuity between different batches of ELISA kits. Will continue to buy from Boster Biological.

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