Anti-NICD Antibody (CST4147)

March 19, 2019

Surgery
University of Pittsburgh
Research Scientist

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Company:

Cell Signaling Technology

Product Name:

Anti-NICD antibody

Catalog Number:

4147

Clone Number:

Ad-iNOS significantly increased activated Notch1 expression in CD24+CD133+ PLC/PRF/5 cells, and the increases were attenuated by TAPI-2 (20 μM or 50 μM, 6 h) in a dose-dependent manner.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates

Primary Incubation

1/1000

Blocking Agent

5% milk

Secondary Incubation

1/2000

Tertiary Incubation

Room temperature 1 hour

Detection

ECL

Results Summary

Ad-iNOS significantly increased activated Notch1 expression in CD24+CD133+ PLC/PRF/5 cells, and the increases were attenuated by TAPI-2 (20 μM or 50 μM, 6 h) in a dose-dependent manner.

DOI or PMID #

https://doi.org/10.1073/pnas.1722100115

Additional Notes

Fig. 4C

Image Gallery

Summary

The Good

Worked well

The Bad

N/A

The Bottom Line

Cleaved Notch1 (V1744) (D3B8) Rabbit mAb detects endogenous levels of the Notch1 intracellular domain (NICD) only when released by cleavage between Gly1753 and Val1754 (equivalent to Gly1743/Val1744 of murine notch1).

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