Boster Biological Technology
Mouse Adipsin / CFD / Factor D PicoKine™ ELISA Kit
EK1420
Info:Supplier Page View Product Specs View Product
I used this kit to detect serum Adipsin levels from C57BL/6 mice and its concentrations in the supernatant of cultured 3T3L1 cells.
Detect adipsin levels in serum and supernatant
N/A
The serum need to be diluted 5000-fold. The supernatant need to be diluted 100-fold. Incubate for 10-15 min is enough after adding Color Developing Reagent.
I did this assay twice. Both times were very good. First time I diluted the mouse serum and cell culture supernatant for 100-fold. However, the values of the serum are out of the range of standards. Then, I repeated the assay by diluting the mouse serum for 3000-fold. Much better than the 1st time, but still some values are marginally out of the range of standards. It is recommended that to do a dilution test using small amount of samples. Generally, you can get a very good result just follow the instruction of this product.
Easy to use. Good quality of data.
Wash buffer is provided as powder. Concentrated solution would be more convenient.
It is a pretty good product with a reasonable price.