Stains Well, but Any Changes Are Subtle

Results Summary

DU145 human prostate cancer cell lines were either grown at regular cell culture conditions (5% CO2 at 37C) or in a hypoxia incubator at 5% O2 at 37C for 48 hours. Afterwards, cells were trypsinized and stained with this BV421 CD39 antibody prior to fixation and permeabilization for intracellular targets. Hypoxia is known to induce CD39 expression, but the changes seen at 1:50 dilution for this antibody between the hypoxia condition and the regular culture conditions are very small. There is clearly a small shift towards increased CD39 expression, but it is very subtle. Subsequent experiments using 1:25 and 1:10 dilutions of the antibody led to similar results without any increase in the fluorescence, so 1:50 was used for subsequent experiments.