ß-catenin Antibody for Immunofluorescence

April 12, 2019

Immunology
MSKCC
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-beta Catenin antibody

Catalog Number:

ab32572

We sought to understand tumorgenesis in PyMT model and we stained for β-catenin to investigate its nuclear translocation.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed mouse (C57BL/6) PyMT mammary gland cryosections

Primary Incubation

1 in 200

Blocking Agent

1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum, 2% goat serum

Secondary Incubation

Goat anti-rabbit Alexa Fluor 647 (Thermofisher, A-21245) 1:500

Tertiary Incubation

None

Detection

Images were acquired using an inverted Zeiss LSM880 confocal microscope with an 20x 0.8NA air objective.

Results Summary

Good staining of β-catenin (red), Hoechst in blue.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable & reproducible staining of β-catenin

The Bad

Expensive

The Bottom Line

Good antibody but very pricey.

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