Santa Cruz
Beta-Actin antibody (C4)
sc-47778
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This monoclonal beta-actin antibody works very well. I always use it to probe for beta-actin as loading control.
Western Blot
Cell lysate
1:2000, 4℃, overnight or room temperature for 1 hour
5% milk
1:5000, room temperature, 1 hour
None
ECL
Specific bands were detected at the correct size. The background is usually pretty clean.
N/A
Cheap, high specificity and sensitivity
This is one of the best actin antibodies.