Cell Signaling Technology
Phospho-c-Jun (Ser73)
9164S
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: Phospho-c-Jun Western Blot Antibodies
Our lab is interested in studying the role of inflammatory kinases as a therapeutic target. We perform ELISA and western blot assays to determine the role and pro-inflammatory status of macrophages in response to pharmacological inhibition of these kinases. This antibody worked well to detect p-c-JUN in murine macrophages following LPS activation.
Western Blot
Murine RAW Macrophages
1:1000
Normal Donkey Serum
1:5000 HRP anti Rabbit
None
Chemi
This worked well but the detection wasn't the brightest for the antibody. Titrations are needed for optimal signal/background. No non-specific bands noticed.
N/A
Works well
Titrate for optimal signal
Works well but needs a little help to get good results.