Great and Easy

Pharmacology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

p105/p50

Catalog Number:

12540S

Our lab is interested in studying the role of inflammatory kinases as a therapeutic target. We perform ELISA and western blot assays to determine the role and pro-inflammatory status of macrophages in response to pharmacological inhibition of these kinases. This antibody worked well for detection in RAW murine macrophages following LPS stimulation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine RAW 247

Primary Incubation

1:500

Blocking Agent

Normal Donkey Serum

Secondary Incubation

1:5000 HRP anti Rabbit

Tertiary Incubation

None

Detection

Chemi

Results Summary

This worked well and was easy to use for detection of the p50 target.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well and reliable

The Bad

None noted

The Bottom Line

This was easy to work and gave great results for a time course of activation study in macrophages.

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