Cell Activation Cocktail (with Brefeldin A)

University Health Network
Advanced Diagnostics
Postdoctoral Fellow

Overall

Quality of Results

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Company:

BioLegend

Product Name:

Cell Activation Cocktail (with Brefeldin A)

Catalog Number:

423303

Cell Activation Cocktail (with Brefeldin A) to stimulate cytokine production by T cells. The product works great.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Cell isolate

Protocol Overview

(1) Dilute 500x of Cell Activation Cocktail (with Brefeldin A) in Media. (2) Incubate for 5–6 hr at 37°C. (3) Wash cells and stain for flow cytometry

Tips

Keep cells in a cell culture incubator

Results Summary

Adding Cell Activation Cocktail (with Brefeldin A) stimulates cells to produce cytokines, such as IFNg

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Cell Activation Cocktail (with Brefeldin A) works great

The Bad

None

The Bottom Line

Adding Cell Activation Cocktail (with Brefeldin A) stimulates cells to produce cytokines, such as IFNg.

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