Alexa 647 Anti-Mouse CD3epsilon

March 18, 2019

Advanced Diagnostics
University Health Network
Postdoctoral Fellow

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Company:

BioLegend

Product Name:

Alexa 647 Anti-Mouse CD3epsilon

Catalog Number:

100324

Clone Number:

Use of Alexa 647 Anti-Mouse CD3epsilon for identification of intracardiac T cells during viral myocarditis. The antibody performs well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Cardiac Cells

Primary Incubation

15 minutes 4°C

Blocking Agent

Anti-CD16/CD32

Secondary Incubation

30 minutes 4°C

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Anti-Mouse CD3epsilon Antibody identifies infiltrating T cells in the heart

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody performs well to label T cells

The Bad

This antibody is not very bright in cardiac samples and needs a dump gate

The Bottom Line

Alexa 647 Anti-Mouse CD3epsilon Antibody works well to identify T cells but requires the use of a dump gate (for myeloid cells)

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